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Microbiology: Unit 2
 This page was last updated on 15-Jun-02

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 Self-Tests - Microbiology:
Genetics & Metabolism
C
hoose the best answer for each question.  At the end of the quiz, click on the "Get Score" button and your quiz will automatically be graded.  The correct reponses will appear in the box underneath your score.

This test has 30 questions and covers microbial metabolism, growth and genetics, as well as recombinant DNA and biotechnology.  It is the second of five units in the Microbiology section.  Good luck!

1. Which of the following is not a cause of enzyme denaturization?
pH
substrate concentration
competitive inhibition
temperature increase

2. Which drug competes with para-aminobenzoic acid in folic acid synthesis?
Sulfa drugs
Erythromycin
Nystatin
Dilantin

3. Which of the following drugs does NOT prevent translation in protein synthesis?
Tetracycline
Streptomycin
Chloramphenicol
Amphotericin B

4. Anaerobic respiration occurs in all of the following except?
Clostridium
Streptococcus
Bacillus
Pseudomonas

5. Which of the following does not occur inside the cell membrane?
Aerobic respiration
Fermentation
Anaerobic respiration
None of the above

6. Which of the following is NOT an end product of glycolysis in an anaerobic organism?
Pyruvic acid
ATP
NADH
Acetyl CoA

7. A 6-carbon glucose makes ___ ATPs by aerobic respiration and ___ ATPs by fermentation.
38, 2
2, 38
3, 8
8, 3

8. Which of the following is a final electron (H) acceptor in anaerobic respiration?
Inorganic O2
Inorganic nitrate
Organic carbonate
Lactic acid

9. Which of the following is NOT a requirement for culturing bacteria on culture media?
Knowing the oxygen requirements
Knowing the nutrient requirements
Knowing the pH requirements
Knowing the UV requirements

10. During which phase of the growth curve is cellular reproduction most active?
Lag Phase
Log Phase
Stationary Phase
Death Phase

11. Which of the following terms refers to the transfer of DNA from one cell to another via bacteriophage?
Transformation
Transcription
Transduction
Conjugation

12. Which of the following types of organisms cannot use atmospheric oxygen?
Facultative
Microaerophilic
Obligate aerobic
Air tolerant

13. Which of the following methods of transferring genetic material requires cell-to-cell contact?
Transduction
Conjugation
Transformation
None of the above

14. Which of the following is NOT a termination codon?
AUG
UAA
UGA
UAG

15. If a codon in a sequence of DNA has the bases of TCT, what is the tRNA anticodon?
AGA
UCU
GTG
UGU

16. Which of the following types of mutation causes base pairs to be added or deleted?
Frameshift mutation
Nonsense mutation
Missense mutation
Point mutation

17. Which of the following is NOT a cause of point mutations?
Caffeine
Nitrogen base analogs
Deaminating agents
Benzpyrene

18. Which of the following is NOT a cause of frameshift mutations?
Benzpyrene
Alkalating agents
Aflatoxin
Acridine dye

19. Which of the following organisms does NOT work against the cell wall by preventing the formation of peptidoglycan?
Cephalosporin
Vancomycin
Bacitracin
Chloramphenicol

20. Which of the following enzymes cuts out damaged DNA to control mutations?
Exonuclease
DNA polymerase
Endonuclease
DNA ligase

21. When an Hfr cell conjugates with an F- cell, what is the result?
F+ cell
F' cell
F- cell
Hfr cell

22. In what year was the first artificial chromosome created by the Human Genome Project?
1984
1990
1997
2000

23. Which of the following terms refers to a technique used in directed evolution to make copies of specific DNA molecules?
Polymerase Chain Reaction
Human Genome Amplification
DNA Testing
Replicative Enzyme Grouping

24. Which of the following terms refers to a technique used to fix inherited diseases in the embryo (fertilized egg)?
Polymerase Chain Reaction
Binary Fission
Germline Engineering
Cloning

25. Which of the following refers to a method of regulating a biological pathway?
Glycolysis
Feedback inhibition
Enzymatic reduction
Endonucleosis

26. During which step in protein synthesis is all RNA made?
Elongation
Transcription
Translation
Initiation

27. During which step of transduction does a phage become integrated into the host chromosomes?
Absorption
Penetration
Latency period
Lysogenic cycle

28. Which step in transduction results in a cell being destroyed?
Lysogenic cycle
Absorption
Lytic cycle
Penetration

29. Which of the following is NOT a selective pressure we put into the environment that encourages resistance?
Antimicrobial cleansers
Incorrect prescriptions
Drug non-compliance
Unmedicated animal feed

30. Which of the following terms refers to a product that can bind to the same active site as a substrate?
Feedback inhibitor
Competitive inhibitor
Biological pathogen
Endonuclease

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